Uric acid is degraded by uricase enzyme immobilized in porous Ca-alginate beads. Experiments conducted with different bead sizes result in the following rate data:

a. Determine the effectiveness factor for particle sizes Dp = 0.5 cm and Dp = 0.7 cm. ? ?

b. The following data were obtained for Dp = 0.5 cm at different bulk uric acid concentrations. Assuming negligible liquid film resistance, calculate Vm and Ks for the enzyme. Assume no substrate or product inhibition.

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Bead diameter, Dp (cm) Rate, v (mg/l.h) 0.1 200 0.2 198 0.3 180 0.4 0.5 140 100 0.6 70 0.7 50 0.8 30 S, (mg UA/1) v (mg UA/1-h) 10 10 25 20 50 30 100 40 200 45 250 46 Bead diameter, Dp (cm) Rate, v (mg/l.h) 0.1 200 0.2 198 0.3 180 0.4 0.5 140 100 0.6 70 0.7 50 0.8 30 S, (mg UA/1) v (mg UA/1-h) 10 10 25 20 50 30 100 40 200 45 250 46