The form of the Scat chard equation given in Impact 15.2 applies only when the macromolecule has

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The form of the Scat chard equation given in Impact 15.2 applies only when the macromolecule has identical and independent binding sites. For non-identical independent binding sites, the Scat chard equation is Plot v/rAJ for the following cases.

(a) There are four independent sites on an enzyme molecule and the intrinsic binding constant is K = 1.0 X 107.

(b) There are a total of six sites per polymer. Four of the sites are identical and have an intrinsic binding constant of 1 x 105. The binding constants for the other two sites are 2 x 106?

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