Explain why incoming DNA recognized by a short RNA molecule expressed from the CRISPR region cannot be completely foreign to the cell.
Answer to relevant QuestionsExplain why an Escherichia coli strain that is His− is an auxotroph and one that is Lac− is not. Explain how a generalized transducing particle differs from a specialized transducing particle. How does the insertional inactivation of β-galactosidase allow the presence of foreign DNA in a plasmid vector such as pUC19 to be detected? What is a subunit vaccine and why are subunit vaccines considered a safer way of conferring immunity to viral pathogens than attenuated virus vaccines? How are gene fusions used to investigate gene regulation?
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