Refer to Figure 12.8, which indicates ΔG for each glycolytic reaction under intracellular conditions. Assume that glyceraldehyde-3-phosphate dehy-drogenase was inhibited with iodoacetate, which reacts with its active site cysteine sulfhydryl group. Which glycolytic intermediate would you expect to accumulate most rapidly, and why?

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Glucose The three enzymes that catalyze reactions so highly exergonic as to be virtually irreversible (arrows) are HK subject to allosteric control. G6P F6P PFK FBP DHAP GAP GAP BPG 3PG 2PCG PEP reactions function at or near equilibrium and are freely reversible in vivo PK Pyr Pathway progress