(a) The plasmid pBR322 (4362 base pairs) was isolated and the circular DNA was found to be...

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(a) The plasmid pBR322 (4362 base pairs) was isolated and the circular DNA was found to be underwound, with a superhelix density (σ) of -0.05. How many superhelical turns does this molecule have? 

(b) Suppose that the molecule was overwound, also with a superhelix density of 0.05. How many superhelical turns would this molecule have?

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Biochemistry Concepts And Connections

ISBN: 9780134641621

2nd Edition

Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews

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