Referring to Question 19, could you separate -amylase from bovine serum albumin (MW 66,000) using this column?

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Referring to Question 19, could you separate β-amylase from bovine serum albumin (MW 66,000) using this column?


Question 19

Sephadex G-75 has an exclusion limit of 80,000 molecular weight for globular proteins. If you tried to use this column material to separate alcohol dehydrogenase (MW 150,000) from β-amylase (MW 200,000), what would happen?

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Biochemistry

ISBN: 9781305961135

9th Edition

Authors: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

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