Referring to Question 23, how would you purify protein X using ion-exchange chromatography if it turns out

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Referring to Question 23, how would you purify protein X using ion-exchange chromatography if it turns out the protein is only stable at a pH between 6 and 6.5?


Question 23

Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.

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Biochemistry

ISBN: 9781305961135

9th Edition

Authors: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

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