Rearrange the statements below to produce a flow diagram showing the steps involved in producing bacteria capable of synthesising a human protein such as human growth hormone (hGH).

1. Insert the plasmid into a host bacterium.

2. Isolate mRNA for hGH.

3. Insert the DNA into a plasmid and use ligase to seal the ‘nicks’ in the sugar–phosphate chains.

4. Use DNA polymerase to clone the DNA.

5. Clone the modified bacteria and harvest hGH.

6. Use reverse transcriptase to produce cDNA.

7. Use a restriction enzyme to cut a plasmid vector.