A. What is the sequence of the DNA that was used in the sequencing reaction shown in Figure Q10–9? The four lanes show the products of sequencing reactions that contained ddG (lane 1), ddA (lane 2), ddT (lane 3), and ddC (lane 4). The numbers to the right of the autoradiograph represent the positions of marker DNA fragments of 50 and 116 nucleotides.

B. This DNA was derived from the middle of a cDNA clone of a mammalian protein. Using the genetic code table (see Figure 7−27), can you determine the amino acid sequence of this portion of the protein?

Figure 7−27

Transcribed Image Text:

codons amino acids AGA AGG GCA CGA Ala GGA GGC GCC CGC GCG CGG GAC AAC UGC GAA CAA GGG CAC AUC CUG AAA GCU CGU GAU AAU UGU GAG CAG GGU CAU AUU CUU AAG AUG Arg Asp Asn Cys Glu Lys Met Phe Gin Gly His lle Leu I E Q H M A R D N с UUA UUG G CUA AUA CUC AGC AGU CCA UCA ACA CCC UCC ACC UUC CCG UCG ACG UUU CCU UCU L K F Pro Ser P S GUA GUC UAC GUG ACU UGG UAU GUU Thr Val T Trp W Tyr Y V UAA UAG UGA stop