Assuming that the histone octamer (shown in Figure 5–20) forms a cylinder 9 nm in diameter and 5 nm in height and that the human genome forms 32 million nucleosomes, what volume of the nucleus (6 μm in diameter) is occupied by histone octamers? Volume of a cylinder is πr 2h; volume of a sphere is 4/3 πr 3.) What fraction of the total volume of the nucleus do the histone octamers occupy? How does this compare with the volume of the nucleus occupied by human DNA?

Transcribed Image Text:

linker DNA "beads-on-a-string" form of chromatin H2A NUCLEASE DIGESTS LINKER DNA released nucleosome core particle DISSOCIATION WITH HIGH CONCENTRATION OF SALT histone octamer H2B core histones of nucleosome nucleosome includes ~200 nucleotide pairs of DNA 7 11 nm ins 147-nucleotide-pair DNA double helix DISSOCIATION H3 H4