Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to insert a gene that would not interfere with ampicillin resistance or production of β-galactosidase by the host cell.

Data from Figure 3-24:

Transcribed Image Text:

Xmnl Scal Pvul- Avall Mstl amp Avall BglI Sspl -2000 AatII Eco0109 0 pUC18 (2.69 kb) HgiEII Ndel, HgiEII Narl lacZ 1000, Bgll Mstl Pvul Pvull Polylinker lacl -Pvull AflIII