For protein denaturation, the excess entropy of denaturation is defined as

is the

transition excess heat capacity. The way in which δC ^trs _P can be extracted from differential scanning calorimetry (DSC) data is discussed in Section 4.6 and shown in Figure 4.7. The following DSC data are for a protein mutant that denatures between T₁ = 288 K and T₂ = 318 K. Using the equation for ΔS _den given previously calculate the excess entropy of denaturation. In your calculations, use the dashed curve as the heat capacity base line which defines δC ^trs  _P as shown in Figure 4.8. Assume the molecular weight of the protein is 14000. grams.

You can perform the integration numerically by counting squares.

Figure 4.7

Figure 4.8

Transcribed Image Text:

SC5 dT, where SC5 AS den т 0.418 J K-'g-1 288 298 308 318 Temperature/K Apparent Heat Capacity LEO'O 0.062 0.083 o.125 0.167 0.229 0.334 0.480 0.627 0.836 1.04 1.23 1.38 1.33 1.17 10.940 0.731 0.522 0.376 7910 b.084