Suppose that you are going to analyze attachment and spreading behavior of mesenchymal stem cells on...
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Suppose that you are going to analyze attachment and spreading behavior of mesenchymal stem cells on a surface with 0.05 kPa elasticity. Immunofluorescence staining is going to be conducted at 1st and 10th h after cell seeding. The names of the antibodies and the fluorochromes are given in the table below. Please, design your immunostaining experiment by matching the antibody and the fluorochrome to be able to show the presence of at least two antigens for the same cells at both 1st and 10th h. Antibodies Anti-Vinculin, Anti-actin stress fibers, Anti-paxillin, Anti-integrin Fluorochromes PE (Phycoerythrin), FITC (Fluorescein), Pacific Blue Suppose that you are going to analyze attachment and spreading behavior of mesenchymal stem cells on a surface with 0.05 kPa elasticity. Immunofluorescence staining is going to be conducted at 1st and 10th h after cell seeding. The names of the antibodies and the fluorochromes are given in the table below. Please, design your immunostaining experiment by matching the antibody and the fluorochrome to be able to show the presence of at least two antigens for the same cells at both 1st and 10th h. Antibodies Anti-Vinculin, Anti-actin stress fibers, Anti-paxillin, Anti-integrin Fluorochromes PE (Phycoerythrin), FITC (Fluorescein), Pacific Blue
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This unit provides a protocol for indirect immunofluorescence which is a method that provides information about the locations of specific molecules an... View the full answer
Related Book For
Probability and Statistics
ISBN: 978-0321500465
4th edition
Authors: Morris H. DeGroot, Mark J. Schervish
Posted Date:
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