A geneticist want to isolate the gene from a tick that encodes the anti-coagulant protein found...
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A geneticist want to isolate the gene from a tick that encodes the anti-coagulant protein found in its saliva which prevents human blood clotting, with the purpose of transferring this gene to bacteria and producing the anti-coagulant for medicinal purposes. a) 4. Should the geneticist construct a tick genomic or CDNA library? Motivate fully. b) Which tick tissue will be most suitable as source of the nucleic acids used for library construction? Explain. c) A partial amino acid sequence from the tick anti-coagulant protein is: . Tyr-Met-Ser-Arg-Phe-Val-Tyr-Lys-His-Cys-Met-Leu-Ile-Arg-Thr-Pro . You wish to make a set of DNA probes to screen your tick library for the clones containing the sequence that encodes this protein. Your probes should be 15 nucleotides in length. 2 A geneticist want to isolate the gene from a tick that encodes the anti-coagulant protein found in its saliva which prevents human blood clotting, with the purpose of transferring this gene to bacteria and producing the anti-coagulant for medicinal purposes. a) 4. Should the geneticist construct a tick genomic or CDNA library? Motivate fully. b) Which tick tissue will be most suitable as source of the nucleic acids used for library construction? Explain. c) A partial amino acid sequence from the tick anti-coagulant protein is: . Tyr-Met-Ser-Arg-Phe-Val-Tyr-Lys-His-Cys-Met-Leu-Ile-Arg-Thr-Pro . You wish to make a set of DNA probes to screen your tick library for the clones containing the sequence that encodes this protein. Your probes should be 15 nucleotides in length. 2
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