An experimental approach is planned with the aim to express a recombinant protein in E coli followed by a purification step using an anti 6X His tag antibody However, for the protein to be used as a therapeutic, the His6 tag needs to be removed Briefly explain how this could be achieved and how th...

To achieve the removal of the His6tag from the recombinant protein expressed in E coli a cleavage site for a specific protease can be introduced betwe ...

An experimental approach is planned with the aim to express a recombinant protein in E. coli...

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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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An experimental approach is planned with the aim to express a recombinant protein in E. coli...

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Fundamentals of biochemistry Life at the Molecular Level

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