For a protein containing no -sheet secondary structure the CD spectrum gives [] 222 = -29,750 deg.cm
Question:
For a protein containing no -sheet secondary structure the CD spectrum gives [Ө] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra that 100% helix has a [Ө] 222 = -36,000 deg.cm 2 .decimole -1 and 100% coil has a [Ө] 222 = +3,000 deg.cm 2 .decimole -1 .
a). What is the percentage alpha-helical content of the protein?
b.) The protein and a short duplex oligonucleotide are added at an equimolar concentration in a buffer containing 100 mM NaCl and then in a buffer containing 1 M NaCl, the two samples are analyzed by CD. The spectra in the 250 to 280 nm region differ considerably between the samples but the spectrums in the 200 – 230 nm range are similar. What hypothesis can you form concerning the protein
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews