The reaction below proceeds with a direct attack by a water molecule on the substrate, but...
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The reaction below proceeds with a direct attack by a water molecule on the substrate, but the hydrolysis could be expected to proceed through two possible mechanisms. Draw two possible mechanisms for the reaction run in H182OH218O. Trace the progress of the 180180 'label' throughout each mechanism to see where it ends up: this should indicate to you how the two mechanisms could be (and in fact were!) distinguished experimentally. ADP OP H20 AMP \OH „OH HO НО OH HỌ The reaction below proceeds with a direct attack by a water molecule on the substrate, but the hydrolysis could be expected to proceed through two possible mechanisms. Draw two possible mechanisms for the reaction run in H182OH218O. Trace the progress of the 180180 'label' throughout each mechanism to see where it ends up: this should indicate to you how the two mechanisms could be (and in fact were!) distinguished experimentally. ADP OP H20 AMP \OH „OH HO НО OH HỌ
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Auditing and Assurance Services
ISBN: 978-0077862343
6th edition
Authors: Timothy Louwers, Robert Ramsay, David Sinason, Jerry Straws
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