Identify all the restriction sites that cut in the Multiple Cloning Site. Pull all the R.E cutting sites (search NEB). Note also the location on the plasmid (site#) and whether it a blunt cutter or a cohesive staggered cutter. Note the temperature the enzyme works best at it, the duration and if there is a stop the reaction protocol. Separate them into tables with columns. Search if any of these enzymes cut elsewhere on the plasmid. Why not? Identify the different regions of the plasmid. What are the functions of these regions? If this was a vector used in yeast or C. elegans eukaryotic expression, what would be the required gene regions for transformation verification and expression.

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Restriction sites in the Multiple Cloning Site Enzyme Recognition Sequence Location site Cutter Type ... View the full answer