Respiration Studies in Isolated Mitochondria Cellular respiration can be studied in isolated mito- chondria by measuring...
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Respiration Studies in Isolated Mitochondria Cellular respiration can be studied in isolated mito- chondria by measuring oxygen consumption under different conditions. If 0.01 M sodium malonate is added to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops and a metabolic intermediate accumulates. (a) What is the structure of this intermediate? (b) Explain why it accumulates. (e) Explain why oxygen consumption stops. (d) Aside from removal of the malonate, how can this inhibition of respiration be overcome? Explain. Respiration Studies in Isolated Mitochondria Cellular respiration can be studied in isolated mito- chondria by measuring oxygen consumption under different conditions. If 0.01 M sodium malonate is added to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops and a metabolic intermediate accumulates. (a) What is the structure of this intermediate? (b) Explain why it accumulates. (e) Explain why oxygen consumption stops. (d) Aside from removal of the malonate, how can this inhibition of respiration be overcome? Explain.
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Related Book For
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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