The following data came from a reversed-phase HPLC separation on a 10 cm column with a...
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The following data came from a reversed-phase HPLC separation on a 10 cm column with a 1.0 mL per minute flow rate and a mobile phase composed of 74% water: 26% acetonitrile. Nonretained A B Retention time, min 1.45 3.00 3.50 5.0 6.0 Width of peak base, min 0.25 0.29 Calculate the number of theoretical plates based on the data for either A, B, C or D. What is the resolution between the critical pair of peaks? 0.42 0.48 What is the selectivity factor between the critical pair? Answer Answer Answer The following data came from a reversed-phase HPLC separation on a 10 cm column with a 1.0 mL per minute flow rate and a mobile phase composed of 74% water: 26% acetonitrile. Nonretained A B Retention time, min 1.45 3.00 3.50 5.0 6.0 Width of peak base, min 0.25 0.29 Calculate the number of theoretical plates based on the data for either A, B, C or D. What is the resolution between the critical pair of peaks? 0.42 0.48 What is the selectivity factor between the critical pair? Answer Answer Answer
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Cornerstones of Financial and Managerial Accounting
ISBN: 978-1111879044
2nd edition
Authors: Rich, Jeff Jones, Dan Heitger, Maryanne Mowen, Don Hansen
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