Suggest a reason why the proofreading step in protein synthesis takes place at the level of amino acid activation rather than that of codon–anticodon recognition.
Answer to relevant QuestionsIdentify the following by describing their functions: EF-G, EF-Tu, EF-Ts, EF-P, and peptidyl transferase. What is the energy cost per amino acid in prokaryotic protein synthesis? Relate this to low entropy. Protein synthesis takes place much more slowly in eukaryotes than in prokaryotes. Suggest a reason why this is so. What are sticky ends? What is their importance in recombinant DNA technology? When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?
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