Suppose you are given the task of constructing a plasmid expression vector suitable for molecular cloning in an organism of industrial interest. List the characteristics such a plasmid should have. List the steps you would use to create such a plasmid.
Answer to relevant QuestionsSuppose you have just determined the DNA base sequence for an especially strong promoter in Escherichia coli and you are interested in incorporating this sequence into an expression vector. Describe the steps you would use. ...Describe the steps for determining an SSU phylogeny of three bacteria you have isolated from nature. What is measured in FAME analyses? Why is sequence alignment necessary prior to phylogenetic tree construction? What are the major fermentation products of the following bacteria: Lactobacillus, Clostridium, Propionibacterium, Escherichia?
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