(a) Why is it illogical to use a thin stationary phase (0.2 m) in a wide-bore (0.53-mm)...
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(b) Consider a narrow-bore (0.25 mm diameter), thin-film (0.10 μm) column with 5 000 plates per meter. Consider also a wide-bore (0.53 mm diameter), thick-film (5.0 μm) column with 1 500 plates per meter. The density of stationary phase is approximately 1.0 g/mL. What mass of stationary phase is in each column in a length equivalent to one theoretical plate? How many nanograms of analyte can be injected into each column if the mass of analyte is not to exceed 1.0% of the mass of stationary phase in one theoretical plate?
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a A thin stationary phase permits rapid equilibration of analyte between the mobile and stationary p...View the full answer
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