How can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a protein?
Answer to relevant QuestionsWhat is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography? An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino ...Determine the values of KM and Vmax for the de-carboxylation of a β-keto acid given the following data. Substrate Concentration (mol L–1) Velocity (mM ...Where do lines intersect on a Lineweaver–Burk plot showing competitive inhibition? On a Lineweaver–Burk plot showing noncompetitive inhibition? What features distinguish enzymes that undergo allosteric control from those that obey the Michaelis–Menten equation?
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