In reverse-phase HPLC, the stationary phase is nonpolar and the mobile phase is a polar solvent at

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In reverse-phase HPLC, the stationary phase is nonpolar and the mobile phase is a polar solvent at neutral pH.

Which of the three amino acids in Question 28 will move fastest on a reverse-phase HPLC column? Which one will move the slowest?

Question 28

An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC.

The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?

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Biochemistry

ISBN: 9781305961135

9th Edition

Authors: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

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Question Posted: Dec 13, 2023 12:48 AM

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In reverse-phase HPLC, the stationary phase is nonpolar and the mobile phase is a polar solvent at

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Biochemistry

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