Consider the tPA process reactor (cultivation) section in Figure 2.14 (a):

(a) For the third cultivator, change the reaction time to three days. To obtain a cell concentration of \(3 \times 10^{6} \mathrm{cell} / \mathrm{mL}\), estimate the liquid volume in the cultivator. What reactor volume would you recommend?

(b) For (a), estimate the \(\mathrm{kg} / \mathrm{batch}\) of air consumed in the third cultivator.

Figure 2.14(a) :-

Transcribed Image Text:

Laboratory Cultivator PA-CHO Cells N, O, CO, Plant Facility Cultivators -70C Hot Water HyQ PF-CHO Media HO- IL 5 days Air CO2 40 L Hot (30 L/batch) 7 days 400 L (300 L/batch) 9.5 days Water" 37C Air 37C HyQ PF-CHO Media 459.5 kg/batch 0.102 kg/batch Media CO 0.02 kg/batch Air Mixing Tank 2.28 kg/batch 37C HO 3,565 kg/batch 5,000 L CO 0.4 kg/batch Air 29.7 kg/batch 2 days CO 5.5 kg/batch Sterilizer (Microfilter) Holding Tank 5,000 L 37C 4C Refrigerant Hot Water Bacteria (a) Reactor section 4C Refrigerant 5,000 L (4,000L/batch) 14 days Centrifuge Holding Tank 5,000 L To Centrifuge