The data in the table are used to create a calibration curve for the determination of...

 

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The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828. The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.

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Line of best fit is y 0028446x 02398 wh... View the full answer