In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the
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In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the culture supernatant after production. A pilot scale bioreactor with a working volume of 250 liters is used for this process and the final biomass concentration is 330 g/L. Considering the separation should be finished in 1 hour. Assuming product yield per biomass (Yp/x) is 0,0006 protein /g biomass, calculate the volume of chromatography resin material required for one bartch purification. Find out the max protein binding capacity of the chromatography resin.
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