With which amino acid sequences would chy-motrypsin be an effective reagent for sequencing the protein from Question 50? Why?
Answer to relevant QuestionsWhat is the basis for the technique called ELISA? What is the advantage to transferring proteins bands form a gel to nitrocellulose during the western blot? If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your ...Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0. For an enzyme that displays Michaelis–Menten kinetics, what is the reaction velocity, V (as a percentage of Vmax), observed at the following values? (a) [S] 5 KM (b) [S] 5 0.5KM (c) [S] 5 0.1KM (d) [S] 5 2KM (e) [S] 5 10KM
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