Question: Which enzymes or chemicals would you choose to use to
Which enzymes or chemicals would you choose to use to cut the protein from Question 50? Why?
Answer to relevant QuestionsWith which amino acid sequences would chy-motrypsin be an effective reagent for sequencing the protein from Question 50? Why? What is an advantage of using an ELISA in-stead of a protein microarray to study a proteome? What is a disad-vantage? You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate pre-cipitation as the next step. Knowing ...How does HPLC differ from ion-exchange chromatography? Define steady state, and comment on the relevance of this concept to theories of enzyme reactivity.
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