Which enzymes or chemicals would you choose to use to cut the protein from Question 50? Why?
Answer to relevant QuestionsWith which amino acid sequences would chy-motrypsin be an effective reagent for sequencing the protein from Question 50? Why? What is an advantage of using an ELISA in-stead of a protein microarray to study a proteome? What is a disad-vantage? You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate pre-cipitation as the next step. Knowing ...How does HPLC differ from ion-exchange chromatography? Define steady state, and comment on the relevance of this concept to theories of enzyme reactivity.
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