Question: A band from a column eluted at 0.66 mL/min has a width at half-height, w1/2, of 39.6 s. The sample was applied as a sharp
A band from a column eluted at 0.66 mL/min has a width at half-height, w1/2, of 39.6 s. The sample was applied as a sharp plug with a volume of 0.40 mL, and the detector volume is 0.25 mL. Find the variances introduced by injection and detection. What would w1/2 be if the only broadening occurred on the column?
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