As mentioned in solved problem S2, origins of transfer can be located in many different locations, and their direction of transfer can be clockwise or counterclockwise. Let's suppose a researcher conjugated six different Hfr strains that were thr+ leu+ tons strr azis lac+ gal+ pro+ met+ to an F- strain that was thr- leu- tonr strs azis lac- gal- pro- met-, and obtained the following results: Strain Order of Gene Transfer

Draw a circular map of the E. coli chromosome and describe the locations and orientations of the origins of transfer in these six Hfr strains.

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Strain Order of Gene Transfer 1 ton azi leu thrmet str' gal lac pro 2 eu azi ton pro lac gal str met thr 3 lac gal str met thr leu azi ton pro 4 leu thr met str gal lac pro ton azi 5 ton pro lac gal str metthr leu azi met str gal lac pro ton azi' leu thr