Assume that k2 kcat for a highly purified enzyme for which you seek to determine G Using the Arrhenius equation, k Ae G RT, where k is a reaction rate constant and A is a constant, you can determine the activation energy to a good approximation (a) Derive a mathematical relationship that will allow you to calculate G and show a graph that can used with experimental data Hint Convert the Arrhenius equation into a linear form, in which the constant A need not be known beforehand (b) What are the easily measurable parameters that you can obtain (c) What assumptions do you need to make in order to obtain a good approximation of the activation energy

Question: Assume that k2 = kcat for a highly purified enzyme for which you seek to determine G. Using the Arrhenius equation, k = Ae-G/RT, where

Assume that k2 = kcat for a highly purified enzyme for which you seek to determine ΔG‡. Using the Arrhenius equation, k = Ae-ΔG‡/RT, where k is a reaction rate constant and A is a constant, you can determine the activation energy to a good approximation.
(a) Derive a mathematical relationship that will allow you to calculate ΔG‡ and show a graph that can used with experimental data. Hint: Convert the Arrhenius equation into a linear form, in which the constant A need not be known beforehand.
(b) What are the easily measurable parameters that you can obtain?
(c) What assumptions do you need to make in order to obtain a good approximation of the activation energy?

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