Consider a negatively charged protein adsorbed on an anionexchange gel at pH 8. (a) How will a
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(a) How will a gradient of eluent pH (from pH 8 to some lower pH) be useful for eluting the protein? Assume that the ionic strength of the eluent is kept constant.
(b) How would a gradient of ionic strength (at constant pH) be useful for eluting the protein?
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a As pH is lowered the protein becomes protonated ...View the full answer
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