Who invented the method of DNA recombination to create genetically engineered organisms in 1973? James Watson and Francis Crick Craig Venter and Francis Collins Paul Berg, Herbert Boyer, Annie Chang, and Stanley Cohen Rosalind Franklin and Maurice Wilkins

Paul Berg, Herbert Boyer, Annie Chang, and Stanley Cohen

_____ detect and cut DNA at particular sequences known as _____, used to introduce new genetic sequences into a genome. Restriction enzymes, restriction sites Helicases, replication forks Ligases, ligation sites Polymerases, promoters

Restriction enzymes, restriction sites

What are the four major steps in rDNA technology? Sequencing, Cloning, Expression, Purification Replication, Transcription, Translation, Mutation Isolation, Restriction digestion, Ligation, Transformation Denaturation, Annealing, Extension, Amplification

Isolation, Restriction digestion, Ligation, Transformation

Recombinant DNA technology

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Biology - Biotechnology

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Cards in this deck(25)

_____ is a synthetic method of genetic alteration that involves adding a new strand not found in nature.

Who invented the method of DNA recombination to create genetically engineered organisms in 1973?

rDNA technology operates on the same theory as the _____, which can offer defense against a variety of viruses.

_____ detect and cut DNA at particular sequences known as _____, used to introduce new genetic sequences into a genome.

_____ is the enzyme used to close the open ends of DNA fragments created by restriction enzymes.

DNA recombinant technology is used in _____ to develop GMOs and agricultural products with desirable traits.

What are the four major steps in rDNA technology?

In rDNA technology, isolation of genetic material involves obtaining a DNA fragment from a _____, genomic library, or cDNA library.

A _____ is any DNA molecule that can replicate inside the host, including plasmids, bacteriophages, and cosmids.

The DNA fragment containing the gene of interest is cut using an enzyme called _____, which acts like scissors.

The DNA fragment is inserted into the open plasmid with the help of _____, which acts like glue.

_____ is the process of DNA imbibition by cells, tissues, or organs.

Which of the following is a physical gene transfer method?

Which of the following is a chemical gene transfer method?

What are the three important applications in Recombinant DNA technology?

What is the enzyme used for joining DNA ends?

What is the term used to describe enzymes that detect and cut DNA at a particular sequence?

What is a transgenic organism?

A _____ is a DNA molecule used to transfer a foreign DNA fragment into a host cell for replication and expression.

In what way/s is recombinant DNA technology useful? a. to produce enzymes for food processing b. all of these c. to produce recombinant proteins for vaccines d. to improve crop yields and increase resistance to pests and diseases

How is the gene of interest spliced into a vector?

Who were the scientists from the University of California San Francisco that discovered the recombinant DNA technique?

What is the correct order of steps in recombinant DNA technology?

What is recombinant DNA technology?

The process of introducing the recombinant DNA molecule into a host cell is typically done using a process called _____

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