Question: A knowledge of molar absorptivities is particularly important in biochemistry, where UV spectroscopy can provide an extremely sensitive method of analysis. For example, imagine that

A knowledge of molar absorptivities is particularly important in biochemistry, where UV spectroscopy can provide an extremely sensitive method of analysis. For example, imagine that you wanted to determine the concentration of vitamin A in a sample. If pure vitamin A has λ max 325 (ε = 50,100), what is the vitamin A concentration in a sample whose absorbance at 325 nm is A 0.735 in a cell with a path length of 1.00 cm?

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