Question: NMR spectroscopy may be used to determine the equilibrium constant for dissociation of a complex between a small molecule, such as an enzyme inhibitor I,

NMR spectroscopy may be used to determine the equilibrium constant for dissociation of a complex between a small molecule, such as an enzyme inhibitor I, and a protein, such as an enzyme E: El→ E + I K[ = [E] [IJ/[EI]

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