Shown below are the biochemical fractionation and purification of an enzyme. Fill in the table on the next page with the values for specific activity and fold purification, and answer the accompanying questions.
(a) Which step provides the greatest purification?
(b) Is the enzyme pure? How can purity be assessed?

Transcribed Image Text:

Total Protein (mg) Activity (nkat)Specific Fold Purification Activity (nkat/mg) Crude extract 50,000 10,000,000 Ammonium sulfate precipitation 5,000 3,750,000 DEAE-cellulose, stepwise Kcl gradient 500 500,000 DEAE-cellulose, KC1 linear gradient 250 500,000 Gel filtration 25 250,000 Substrate affinity chromatography 100,000