If you want to purify a DNA-binding protein from a crude mixture of proteins at pH 7,
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If you want to purify a DNA-binding protein from a crude mixture of proteins at pH 7, should you use a DEAE-cellulose or a CM-cellulose column (see Figure 5.A5)? Briefly explain your reasoning.
Figure 5.A5.
Transcribed Image Text:
H Weak anion exchanger (DEAE) Weak cation exchanger (CM) (X) R Xxn R R Strong anion exchanger ("Q") 6=0 Strong cation exchanger ("S")
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Related Book For
Biochemistry Concepts And Connections
ISBN: 9780134641621
2nd Edition
Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews
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