Give the approximate molecular masses of an immunoglobulin G molecule analyzed by (a) Gel filtration chromatography, (b)
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Give the approximate molecular masses of an immunoglobulin G molecule analyzed by
(a) Gel filtration chromatography,
(b) SDS-PAGE,
(c) SDS-PAGE in the presence of 2-mercaptoethanol.
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The approximate molecular masses of an immunoglobulin G IgG molecule analyzed by different techniques are as follows a Gel Filtration Chromatography Gel filtration chromatography separates molecules based on their size and shape IgG is a relatively large protein and its molecular mass when analyzed by gel filtration chromatography is approximately 150000 to 160000 daltons Da This is based on the size of the protein and how it elutes through the column b SDSPAGE Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis SDSPAGE is a technique ...View the full answer
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Related Book For
Fundamentals Of Biochemistry Life At The Molecular Level
ISBN: 9781118918401
5th Edition
Authors: Donald Voet, Judith G Voet, Charlotte W Pratt
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