Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to
Question:
Refer to the diagram of pUC18 (Fig. 3-24) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.
Data from Figure 3-24:
Transcribed Image Text:
Xmnl Scal Pvul- Avall Mstl amp Avall BglI Sspl -2000 AatII EcoO109 0 pUC18 (2.69 kb) HgiEII Ndel, HgiEII Narl lacZ 1000, Bgll Mstl Pvul Pvull Polylinker lacl -Pvull AflIII
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Related Book For
Fundamentals Of Biochemistry Life At The Molecular Level
ISBN: 9781118918401
5th Edition
Authors: Donald Voet, Judith G Voet, Charlotte W Pratt
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