Using the data in Table 3-2, identify restriction enzymes that
(a) Produce blunt ends

(b) Recognize and cleave the same sequence (called isoschizomers)

(c) Produce identical sticky ends.

Data from Table 3-2:

Transcribed Image Text:

TABLE 3-2 Recognition and Cleavage Sites of Some Restriction Enzymes Recognition Sequence Enzyme Alul BamHI BglII EcoRI EcoRII EcoRV Haell HaeIII HindIII Hpall Mspl Pstl Pvull Sall Taql Xhol AGICT GIGATCC A GATCT GIAATTC ICCAGG GAT ATC RGCGCIY GG CC ALAGCTT CICGG CICGG CTGCAIG CAGICTG GTCGAC TICGA CITCGAG Microorganism Arthrobacter luteus Bacillus amyloliquefaciens H Bacillus globigii Escherichia coli RY 13 Escherichia coli R245 Escherichia coli J62 pLG74 Haemophilus aegyptius Haemophilus aegyptius Haemophilus influenzae Ra Haemophilus parainfluenzae Moraxella species Providencia stuartii 164 Proteus vulgaris Streptomyces albus G Thermus aquaticus Xanthomonas holcicola