A test TLC plate (silica) using a 1 : 3 mixture of CH 3 CN:H 2 O
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A test TLC plate (silica) using a 1 : 3 mixture of CH3CN:H2O as eluent shows that two compounds have Rf values of 0.52 and 0.15:
You plan to use column LC to separate a mixture of the two compounds. How can you attempt to ensure that their behaviour on the column will closely mimic that on the TLC plate?
Transcribed Image Text:
R₁ = 0.52 R = 0.15 -T-----
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The silica layer of the TLC plate should closely match the stationary phase This is so because the stationary phase which interacts with the compounds ...View the full answer
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