In the polyproline spectroscopic ruler experiment shown in Figure 25.19, the FRET pair employed is comprised of

In the polyproline €œspectroscopic ruler€ experiment shown in Figure 25.19, the FRET pair employed is comprised of the fluorescent dyes Alexa Fluor 488 (excited-state lifetime of 4.1 ns) and Alexa Fluor 594. For this FRET pair R₀= 5.4 nm. The distance between the FRET pair ranges from 2.0 nm for Pro6 to 12.5 nm for Pro40. Calculate the variation in the energy transfer rate for r = 2.0, 7.0, and 12.0 nm. D_oyour results agree with the trend evident in Figure 25.19?

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AngA 1.0 1.0 Pro20 Pro27 Pro33 Pro40 0.8 0.8 0.6 - 0.6 Pro11 Pro14 Pro6 0.4 0.4 0.2 0.2 0.0 0.0 Relative event frequency