A DNA template plus primer with the structure

(where P = a phosphate group) is placed in an in vitro DNA synthesis system (Mg²⁺, an excess of the four deoxyribonucleoside triphosphates, etc.) containing a mutant form of E. coli DNA polymerase I that lacks exonuclease activity. The polymerase and exonuclease activities of this aberrant enzyme are identical to those of normal E. coli DNA polymerase I. It simply has no exonuclease activity.
(a) What will be the structure of the final product?
(b) What will be the first step in the reaction sequence?

Transcribed Image Text:

3' P-TGCGAATTAGCGACAT–P5' 5 Р —АТCGGTACGACGCTTAAC—ОН 3'