Assume that you have discovered a new strain of E. coli that has a mutation in the

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Assume that you have discovered a new strain of E. coli that has a mutation in the lac operator region that causes the wild-type repressor protein to bind irreversibly to the operator. You have named this operator mutant Osb for “superbinding” operator. 

(a) What phenotype would a partial diploid of genotype I+OsbZ-Y+/I+O+Z+Y- have with respect to the synthesis of the enzymes β-galactosidase and β-galactoside permease? 

(b) Does your new Osb mutation exhibit cis or trans dominance in its effects on the regulation of the lac operon?

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Principles of Genetics

ISBN: 978-1119142287

7th edition

Authors: D. Peter Snustad, Michael J. Simmons

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