Mutations nrd 11 (gene nrd B, encoding the beta subunit of the enzyme ribonucleotide reductase), am M69 (gene 63, encoding a protein that aids tail-fiber attachment), and nd 28 (gene denA, encoding the enzyme endonuclease II) are known to be located between gene 31 and gene 32 on the bacteriophage T4 chromosome. Mutations am N54 and am A453 are located in genes 31 and 32, respectively. Given the three-factor cross data in the following table, what is the linear order of the five mutant sites?

^a All recombination frequencies are given as

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Three-Factor Cross Data % Recombination Cross 2.6 1. ат A453—ат М69 Х mrd 11 4.2 2. ат А453—ат М69 X пrd 11 2.5 3. ат A453—ат M69 X nd 28 3.5 4. ат A453—nd 28 X ат M69 2.9 5. ат A453—пrd 11 x nd 28 2.1 6. ат А453—пd 28 X пrd 11 3.5 7. аm N54—ат М69 Х пrd 11 1.9 8. ат N54 —пrd 11 x ат М69 1.7 9. ат N54 —nd 28 х ат М69 2.7 10. ат N54 —aт M69 X nd 28 2.9 11. ат N54—nd 28 X пrd 11 1.9 12. am N54–nrd 11 × nd 28 (wild type progeny) 2 total progeny х 100.