Mutations nrd 11 (gene nrd B, encoding the beta subunit of the enzyme ribonucleotide reductase), am M69 (gene 63, encoding a protein that aids tail-fiber attachment), and nd 28 (gene denA, encoding the enzyme endonuclease II) are known to be located between gene 31 and gene 32 on the bacteriophage T4 chromosome. Mutations am N54 and am A453 are located in genes 31 and 32, respectively. Given the three-factor cross data in the following table, what is the linear order of the five mutant sites?

^a All recombination frequencies are given as

Three-Factor Cross Data % Recombination Cross 2.6 1. A453 69 mrd 11 4.2 2. 453 69 X rd 11 2.5 3. A453 M69 X nd 28 3.5 4. A453nd 28 X M69 2.9 5. A453rd 11 x nd 28 2.1 6. 453d 28 X rd 11 3.5 7. m N54 69 rd 11 1.9 8. N54 rd 11 x 69 1.7 9. N54 nd 28 69 2.7 10. N54 a M69 X nd 28 2.9 11. N54nd 28 X rd 11 1.9 12. am N54nrd 11 nd 28 (wild type progeny) 2 total progeny 100.