The Boston barberry is an imaginary plant with a diploid chromosome number of 4, and Boston barberry cells are easily grown in suspended cell cultures. 3H-thymidine was added to the culture medium in which a G1-stage cell of this plant was growing. After one cell ONLINE_generation of growth in 3H-thymidine-ontaining medium, colchicine was added to the culture medium. The medium now contained both 3H-thymidine and colchicine. After two €œgenerations€ of growth in 3H-thymidine- containing medium (the second €œgeneration€ occurring in the presence of colchicine as well), the two progeny cells (each now containing eight chromosomes) were transferred to culture medium containing nonradioactive thymidine (3H-thymidine) plus colchicine.
Note that a €œgeneration€ in the presence of colchicine consists of a normal cell cycle€™s chromosomal duplication but no cell division. The two progeny cells were allowed to continue to grow, proceeding through the €œcell cycle,€ until each cell contained a set of metaphase chromosomes that looked like the following.

If autoradiography were carried out on these metaphase chromosomes (four large plus four small), what pattern of radioactivity (as indicated by silver grains on the autoradiograph) would be expected? (Assume no recombination between DNA molecules.)