Below are data compiled from kinetic analysis of an enzyme isolated from blueberry plant leaves. The enzyme
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Below are data compiled from kinetic analysis of an enzyme isolated from blueberry plant leaves. The enzyme catalyzes the hydrolysis of esters such as methyl benzoate. Use the data to create a Michaelis-Menton plot and Line weaver-Burke plot in Excel. Determine K M and V max from the data. The data were obtained by measuring the rate of disappearance of substrate.
[Methyl Benzoate] in micro M | v o in micro M/min |
3.7 | 10 |
13.0 | 30 |
39.0 | 63 |
79.0 | 87 |
230 | 116 |
400 | 122 |
Related Book For
Practicing Statistics Guided Investigations for the Second Course
ISBN: 978-0321586018
1st edition
Authors: Shonda Kuiper, Jeff Sklar
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