Transferrin is the iron-transport protein found in blood. It has a molecular weight of 000 and carries two Fe ⁺³ ions. Desferrioxamine B is a potent iron chelator used to treat patients with iron overload. It has a molecular weight of about 650 and can bind one Fe ⁺³ . Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. The molar absorptivities of these compounds (saturated with iron) at two wavelengths are given in the table. Both compounds are colorless (no visible absorption) in the absence of iron.

A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cuvet. Calculate the coneentrationmi trailsceirinin milligrams per milliliter and the concentration of iron in micrograms per milliliter.

Transcribed Image Text:

λ (nm) 428 470 & (M-1cm-1) Transferrin Desferrioxamine 3540 4170 2730 2290 λ (nm) 428 470 & (M-1cm-1) Transferrin Desferrioxamine 3540 4170 2730 2290

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BeerLambert law is A l C where A Absorbance at 470 ... View the full answer